Dependence of DNA Denaturation on G.C Content and on Salt Concentration

Dependence of DNA Denaturation

The greater the G.C content, the higher the temperature must be to denature the DNA strand. DNA from different sources was dissolved in solutions of low (red line) and high (green line) concentrations of salt at pH 7.0. The points represent the temperature at which the DNA denatured, graphed against the G.C content.